HybridSPAdes: an algorithm for hybrid assembly of short and long reads.

ABSTRACT:

Motivation

Recent advances in single molecule real-time (SMRT) and nanopore sequencing technologies have enabled high-quality assemblies from long and inaccurate reads. However, these approaches require high coverage by long reads and remain expensive. On the other hand, the inexpensive short reads technologies produce accurate but fragmented assemblies. Thus, a hybrid approach that assembles long reads (with low coverage) and short reads has a potential to generate high-quality assemblies at reduced cost.

Results

We describe hybridSPAdes algorithm for assembling short and long reads and benchmark it on a variety of bacterial assembly projects. Our results demonstrate that hybridSPAdes generates accurate assemblies (even in projects with relatively low coverage by long reads

SUBMITTER: Antipov D 

PROVIDER: S-EPMC4907386 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature