Dataset Information

Characterization of mycobacteria and mycobacteriophages isolated from compost at the Sao Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U.

ABSTRACT:

Background

A large collection of sequenced mycobacteriophages capable of infecting a single host strain of Mycobacterium smegmatis shows considerable genomic diversity with dozens of distinctive types (clusters) and extensive variation within those sharing evident nucleotide sequence similarity. Here we profiled the mycobacterial components of a large composting system at the São Paulo zoo.

Results

We isolated and sequenced eight mycobacteriophages using Mycobacterium smegmatis mc(2)155 as a host. None of these eight phages infected any of mycobacterial strains isolated from the same materials. The phage isolates span considerable genomic diversity, including two phages (Barriga, Nhonho) related to Subcluster A1 phages, two Cluster B phages (Pops, Subcluster B1; Godines, Subcluster B2), three Subcluster F1 phages (Florinda, Girafales, and Quico), and Madruga, a relative of phage Patience with which it constitutes the new Cluster U. Interestingly, the two Subcluster A1 phages and the three Subcluster F1 phages have genomic relationships indicating relatively recent evolution within a geographically isolated niche in the composting system.

Conclusions

We predict that composting systems such as those used to obtain these mycobacteriophages will be a rich source for the isolation of additional phages that will expand our view of bacteriophage diversity and evolution.

SUBMITTER: Lima-Junior JD

PROVIDER: S-EPMC4912749 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Publications

Characterization of mycobacteria and mycobacteriophages isolated from compost at the São Paulo Zoo Park Foundation in Brazil and creation of the new mycobacteriophage Cluster U.

Lima-Junior James Daltro JD Viana-Niero Cristina C Conde Oliveira Daniel V DV Machado Gabriel Esquitini GE Rabello Michelle Cristiane da Silva MC Martins-Junior Joaquim J Martins Layla Farage LF Digiampietri Luciano Antonio LA da Silva Aline Maria AM Setubal João Carlos JC Russell Daniel A DA Jacobs-Sera Deborah D Pope Welkin H WH Hatfull Graham F GF Leão Sylvia Cardoso SC

BMC microbiology 20160617 1

<h4>Background</h4>A large collection of sequenced mycobacteriophages capable of infecting a single host strain of Mycobacterium smegmatis shows considerable genomic diversity with dozens of distinctive types (clusters) and extensive variation within those sharing evident nucleotide sequence similarity. Here we profiled the mycobacterial components of a large composting system at the São Paulo zoo.<h4>Results</h4>We isolated and sequenced eight mycobacteriophages using Mycobacterium smegmatis mc ...[more]

PMID: 27316672

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