Dataset Information

Metabolic and Hormonal Response to a Feed-challenge Test in Lean and Overweight Dogs.

ABSTRACT:

Background

Obese dogs risk poor life quality, creating a need for increased knowledge of metabolism in overweight dogs.

Objectives

Investigate postprandial metabolic and hormonal responses to a high-fat mixed-meal in dogs and responses of lean versus overweight dogs.

Animals

Twenty-eight healthy intact male Labrador Retrievers were included.

Methods

Prospective observational study. Twelve dogs were grouped as lean (body condition score (BCS 4-5), 10 as slightly overweight (BCS 6), and 6 as overweight (BCS 6.5-8) on a 9-point scale. After an overnight fast, urine and blood samples were collected. Dogs were then fed a high-fat mixed-meal, and blood was collected hourly for 4 hours and urine after 3 hours.

Results

Postprandial concentrations of insulin and glucagon were increased at 1 hour (both P < 0.0001), triglycerides at 2 hours (P < 0.0001), and glucose at 3 hours (P = 0.004); and all remained increased throughout the feed-challenge in all dogs. Postprandial urine cortisol/creatinine ratio was higher than fasting values (P = 0.001). Comparing between groups, there was an overall higher triglyceride response in overweight compared to lean (P = 0.001) and slightly overweight (P = 0.015) dogs. Overweight dogs also had higher fasting cortisol/creatinine ratio compared to lean dogs (P = 0.024).

Conclusions and clinical importance

Postprandial responses of dogs to a high-fat mixed-meal were similar to those previously reported in people. The higher postprandial triglyceride response and fasting cortisol/creatinine ratio in the overweight dogs could be early signs of metabolic imbalance. Thus, although overweight dogs often appear healthy, metabolic alterations might be present.

SUBMITTER: Soder J

PROVIDER: S-EPMC4913616 | biostudies-literature | 2016 Mar-Apr

REPOSITORIES: biostudies-literature

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Publications

Metabolic and Hormonal Response to a Feed-challenge Test in Lean and Overweight Dogs.

Söder J J Wernersson S S Hagman R R Karlsson I I Malmlöf K K Höglund K K

Journal of veterinary internal medicine 20160129 2

<h4>Background</h4>Obese dogs risk poor life quality, creating a need for increased knowledge of metabolism in overweight dogs.<h4>Objectives</h4>Investigate postprandial metabolic and hormonal responses to a high-fat mixed-meal in dogs and responses of lean versus overweight dogs.<h4>Animals</h4>Twenty-eight healthy intact male Labrador Retrievers were included.<h4>Methods</h4>Prospective observational study. Twelve dogs were grouped as lean (body condition score (BCS 4-5), 10 as slightly overw ...[more]

PMID: 26822126

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Project description:Obesity in dogs is an increasing problem and better knowledge of the metabolism of overweight dogs is needed. Identification of molecular changes related to overweight may lead to new methods to improve obesity prevention and treatment. The aim of the study was firstly to investigate whether Nuclear Magnetic Resonance (NMR) based metabolomics could be used to differentiate postprandial from fasting urine in dogs, and secondly to investigate whether metabolite profiles differ between lean and overweight dogs in fasting and postprandial urine, respectively. Twenty-eight healthy intact male Labrador Retrievers were included, 12 of which were classified as lean (body condition score (BCS) 4-5 on a 9-point scale) and 16 as overweight (BCS 6-8). After overnight fasting, a voided morning urine sample was collected. Dogs were then fed a high-fat mixed meal and postprandial urine was collected after 3 hours. Metabolic profiles were generated using NMR and 45 metabolites identified from the spectral data were evaluated using multivariate data analysis. The results revealed that fasting and postprandial urine differed in relative metabolite concentration (partial least-squares discriminant analysis (PLS-DA) 1 comp: R2Y = 0.4, Q2Y = 0.32; cross-validated ANOVA: P = 0.00006). Univariate analyses of discriminant metabolites showed that taurine and citrate concentrations were elevated in postprandial urine, while allantoin concentration had decreased. Interestingly, lean and overweight dogs differed in terms of relative metabolite concentrations in postprandial urine (PLS-DA 1 comp: R2Y = 0.5, Q2Y = 0.36, cross-validated ANOVA: P = 0.005) but not in fasting urine. Overweight dogs had lower postprandial taurine and a trend of higher allantoin concentrations compared with lean dogs. These findings demonstrate that metabolomics can differentiate 3-hour postprandial urine from fasting urine in dogs, and that postprandial urine metabolites may be more useful than fasting metabolites for identification of metabolic alterations linked to overweight. The lowered urinary taurine concentration in overweight dogs could indicate alterations in lipid metabolism and merits further investigation.

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Dataset Information

Metabolic and Hormonal Response to a Feed-challenge Test in Lean and Overweight Dogs.

Background

Objectives

Animals

Methods

Results

Conclusions and clinical importance

Publications

Metabolic and Hormonal Response to a Feed-challenge Test in Lean and Overweight Dogs.

Similar Datasets

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