Capturing the dynamic nascent transcriptome during acute cellular responses: The serum response.

ABSTRACT: Dynamic regulation of gene expression via signal transduction pathways is of fundamental importance during many biological processes such as cell state transitioning, cell cycle progression and stress responses. In this study we used serum stimulation as a cell response paradigm to apply the nascent RNA Bru-seq technique in order to capture early dynamic changes in the nascent transcriptome. Our data provides an unprecedented view of the dynamics of genome-wide transcription during the first two hours of serum stimulation in human fibroblasts. While some genes showed sustained induction or repression, other genes showed transient or delayed responses. Surprisingly, the dynamic patterns of induction and suppression of response genes showed a high degree of similarity, suggesting that these

SUBMITTER: Kirkconnell KS 

PROVIDER: S-EPMC4920201 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature