WW domain binding protein 5 induces multidrug resistance of small cell lung cancer under the regulation of miR-335 through the Hippo pathway.

ABSTRACT:

Background

Our previous study indicated that WW domain binding protein 5 (WBP5) expression was elevated significantly in a drug-resistant cell compared with its parental cell. Nevertheless, its functional role and underlying mechanisms remain unknown.

Methods

In this study, WBP5 was examined in 62 small cell lung cancer (SCLC) patient samples by immunohistochemical technique. Stable WBP5-overexpressed and WBP5-underexpressed cells were further established to assess the role of WBP5 in drug resistance, apoptosis and tumour growth. We also conducted western blot to detect the expression of MST2 and YAP1 and their phosphorylated protein.

Results

The results revealed that WBP5 expression was significantly associated with the shorter survival time in SCLC patients. Upregul

SUBMITTER: Tang R 

PROVIDER: S-EPMC4947702 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature