Identification of disease-specific motifs in the antibody specificity repertoire via next-generation sequencing.

ABSTRACT: Disease-specific antibodies can serve as highly effective biomarkers but have been identified for only a relatively small number of autoimmune diseases. A method was developed to identify disease-specific binding motifs through integration of bacterial display peptide library screening, next-generation sequencing (NGS) and computational analysis. Antibody specificity repertoires were determined by identifying bound peptide library members for each specimen using cell sorting and performing NGS. A computational algorithm, termed Identifying Motifs Using Next- generation sequencing Experiments (IMUNE), was developed and applied to discover disease- and healthy control-specific motifs. IMUNE performs comprehensive pattern searches, identifies patterns statistically enriched in the disease or

SUBMITTER: Pantazes RJ 

PROVIDER: S-EPMC4969583 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature