Next Generation Protein Sequencing

ABSTRACT: NGPS is a method for de-novo, full-length protein sequencing in high throughput. The method is based on cleavage of the protein at semi-random sites by microwave-assisted acid hydrolysis (MAAH), enrichment of LC-MS/MS amenable peptides from the hydrolysate by solid-phase-extraction, LC-MS/MS analysis, de-novo long peptide tag sequencing of resulting peptides and assembly of peptide tags into consensus contigs.

INSTRUMENT(S):

ORGANISM(S): Equus Caballus (horse) Bos Taurus (bovine)

SUBMITTER: Alon Savidor  

LAB HEAD: Yishai Levin

PROVIDER: PXD003804 | Pride | 2017-04-03

REPOSITORIES: Pride