Gravidas with class III obesity: evaluating the abdominal skin microbiota above and below the panniculus (.).

ABSTRACT:

Objective

We sought to compare the skin microbiota above and below the panniculus of third-trimester pregnant women with class III obesity.

Methods

We collected swabs from the anterior panniculus and from the intertriginous area under the panniculus of women with class III obesity (body mass index ≥40 kg/m(2)) carrying a live singleton at ≥28 weeks. DNA was extracted. PCR with primers targeting the 16S rDNA V4 region were used to prepare an amplicon library. PCR products were sequenced (Illumina MiSeq platform). The 16S rDNA sequences were processed, integrated, analyzed and reported using QIIME and in-house developed scripts. Taxonomy was assigned using RDP Classifier (threshold 0.8) against the Greengenes 16S rDNA database.

Results

Twenty women were enrolled. One sa

SUBMITTER: Edwards RK 

PROVIDER: S-EPMC4975423 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature