Design and application of a target capture sequencing of exons and conserved non-coding sequences for the rat.

ABSTRACT:

Background

Target capture sequencing is an efficient approach to directly identify the causative mutations of genetic disorders. To apply this strategy to laboratory rats exhibiting various phenotypes, we developed a novel target capture probe set, TargetEC (target capture for exons and conserved non-coding sequences), which can identify mutations not only in exonic regions but also in conserved non-coding sequences and thus can detect regulatory mutations.

Results

TargetEC covers 1,078,129 regions spanning 146.8 Mb of the genome. We applied TargetEC to four inbred rat strains (WTC/Kyo, WTC-swh/Kyo, PVG/Seac, and KFRS4/Kyo) maintained by the National BioResource Project for the Rat in Japan, and successfully identified mutations associated with these phenotypes, including on

SUBMITTER: Yoshihara M 

PROVIDER: S-EPMC4979189 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature