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Synthesis of a deuterated probe for the confocal Raman microscopy imaging of squalenoyl nanomedicines.

ABSTRACT: The synthesis of ω-di-(trideuteromethyl)-trisnorsqualenic acid has been achieved from natural squalene. The synthesis features the use of a Shapiro reaction of acetone-d 6 trisylhydrazone as a key step to implement the terminal isopropylidene-d 6 moiety. The obtained squalenic acid-d 6 has been coupled to gemcitabine to provide the deuterated analogue of squalenoyl gemcitabine, a powerful anticancer agent endowed with self-assembling properties. The Raman spectra of both deuterated and non-deuterated squalenoyl gemcitabine nanoparticles displayed significant Raman scattering signals. They revealed no differences except from the deuterium peak patterns in the silent spectral region of cells. This paves the way for label-free intracellular trafficking studies of squalenoyl nanomedicines.

SUBMITTER: Buchy E

PROVIDER: S-EPMC4979966 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature

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Synthesis of a deuterated probe for the confocal Raman microscopy imaging of squalenoyl nanomedicines.

Buchy Eric E Vukosavljevic Branko B Windbergs Maike M Sobot Dunja D Dejean Camille C Mura Simona S Couvreur Patrick P Desmaële Didier D

Beilstein journal of organic chemistry 20160606

The synthesis of ω-di-(trideuteromethyl)-trisnorsqualenic acid has been achieved from natural squalene. The synthesis features the use of a Shapiro reaction of acetone-d 6 trisylhydrazone as a key step to implement the terminal isopropylidene-d 6 moiety. The obtained squalenic acid-d 6 has been coupled to gemcitabine to provide the deuterated analogue of squalenoyl gemcitabine, a powerful anticancer agent endowed with self-assembling properties. The Raman spectra of both deuterated and non-deute ...[more]

PMID: 27559365

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