Use of Unamplified RNA/cDNA-Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses.
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ABSTRACT:
SUBMITTER: Kilianski A
PROVIDER: S-EPMC4982148 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Publications
Use of Unamplified RNA/cDNA-Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses.
Kilianski Andy A Roth Pierce A PA Liem Alvin T AT Hill Jessica M JM Willis Kristen L KL Rossmaier Rebecca D RD Marinich Andrew V AV Maughan Michele N MN Karavis Mark A MA Kuhn Jens H JH Honko Anna N AN Rosenzweig C Nicole CN
Emerging infectious diseases 20160815 8
Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezuelan equine encephalitis virus and Ebola virus in 3 hours from sample receipt to data acquisition, demonstrating a fieldable technique for RNA virus characterization. ...[more]
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