Ontology highlight
ABSTRACT:
SUBMITTER: Hall AB
PROVIDER: S-EPMC5026532 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20150521 6240
Sex determination in the mosquito Aedes aegypti is governed by a dominant male-determining factor (M factor) located within a Y chromosome-like region called the M locus. Here, we show that an M-locus gene, Nix, functions as an M factor in A. aegypti. Nix exhibits persistent M linkage and early embryonic expression, two characteristics required of an M factor. Nix knockout with clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 resulted in largely feminized genetic males and ...[more]