Single sample resolution of rare microbial dark matter in a marine invertebrate metagenome.

ABSTRACT: Direct, untargeted sequencing of environmental samples (metagenomics) and de novo genome assembly enable the study of uncultured and phylogenetically divergent organisms. However, separating individual genomes from a mixed community has often relied on the differential-coverage analysis of multiple, deeply sequenced samples. In the metagenomic investigation of the marine bryozoan Bugula neritina, we uncovered seven bacterial genomes associated with a single B. neritina individual that appeared to be transient associates, two of which were unique to one individual and undetectable using certain "universal" 16S rRNA primers and probes. We recovered high quality genome assemblies for several rare instances of "microbial dark matter," or phylogenetically divergent bacteria lacking genomes in r

SUBMITTER: Miller IJ 

PROVIDER: S-EPMC5041132 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature