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Tunable and label-free virus enrichment for ultrasensitive virus detection using carbon nanotube arrays.


ABSTRACT: Viral infectious diseases can erupt unpredictably, spread rapidly, and ravage mass populations. Although established methods, such as polymerase chain reaction, virus isolation, and next-generation sequencing have been used to detect viruses, field samples with low virus count pose major challenges in virus surveillance and discovery. We report a unique carbon nanotube size-tunable enrichment microdevice (CNT-STEM) that efficiently enriches and concentrates viruses collected from field samples. The channel sidewall in the microdevice was made by growing arrays of vertically aligned nitrogen-doped multiwalled CNTs, where the intertubular distance between CNTs could be engineered in the range of 17 to 325 nm to accurately match the size of different viruses. The CNT-STEM significantly improves detection limits and virus isolation rates by at least 100 times. Using this device, we successfully identified an emerging avian influenza virus strain [A/duck/PA/02099/2012(H11N9)] and a novel virus strain (IBDV/turkey/PA/00924/14). Our unique method demonstrates the early detection of emerging viruses and the discovery of new viruses directly from field samples, thus creating a universal platform for effectively remediating viral infectious diseases.

SUBMITTER: Yeh YT 

PROVIDER: S-EPMC5055386 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Tunable and label-free virus enrichment for ultrasensitive virus detection using carbon nanotube arrays.

Yeh Yin-Ting YT   Tang Yi Y   Sebastian Aswathy A   Dasgupta Archi A   Perea-Lopez Nestor N   Albert Istvan I   Lu Huaguang H   Terrones Mauricio M   Zheng Si-Yang SY  

Science advances 20161007 10


Viral infectious diseases can erupt unpredictably, spread rapidly, and ravage mass populations. Although established methods, such as polymerase chain reaction, virus isolation, and next-generation sequencing have been used to detect viruses, field samples with low virus count pose major challenges in virus surveillance and discovery. We report a unique carbon nanotube size-tunable enrichment microdevice (CNT-STEM) that efficiently enriches and concentrates viruses collected from field samples.  ...[more]

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