Carbon extension in peptidylnucleoside biosynthesis by radical SAM enzymes.

ABSTRACT: Nikkomycins and polyoxins are antifungal peptidylnucleoside antibiotics active against human and plant pathogens. Here we report that during peptidylnucleoside biosynthesis in Streptomyces cacaoi and S. tendae, the C5' extension of the nucleoside essential for downstream structural diversification is catalyzed by a conserved radical S-adenosyl-L-methionine (SAM) enzyme, PolH or NikJ. This is distinct from the nucleophilic mechanism reported for antibacterial nucleosides and represents a new mechanism of nucleoside natural product biosynthesis.

SUBMITTER: Lilla EA 

PROVIDER: S-EPMC5069167 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature