Production and verification of a 2^nd generation clonal group of Japanese flounder, Paralichthys olivaceus.

ABSTRACT: Clonal fishes are useful tools in biology and aquaculture studies due to their isogenicity. In Japanese flounder (Paralichthys olivaceus), a group of homozygous clones was created by inducing meiogynogenesis in eggs from a mitogynogenetic homozygous diploid. As the clones reached sexual maturity, meiogynogenesis was again induced in order to produce a 2^nd generation clonal group of Japanese flounder. After 3 months, there were 611 healthy, surviving individuals. Twenty-four microsatellite markers, that covered all the linkage groups of Japanese flounder, were used to identify the homozygosity of the 2^nd generation clones; no heterozygous locus was detected. This indicates that the production of a 2^nd generation clonal group of Japanese flounder was successf

SUBMITTER: Hou J 

PROVIDER: S-EPMC5073307 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature