Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq.

ABSTRACT:

Background

The robustness of ChIP-seq datasets is highly dependent upon the antibodies used. Currently, polyclonal antibodies are the standard despite several limitations: They are non-renewable, vary in performance between lots and need to be validated with each new lot. In contrast, monoclonal antibody lots are renewable and provide consistent performance. To increase ChIP-seq standardization, we investigated whether monoclonal antibodies could replace polyclonal antibodies. We compared monoclonal antibodies that target five key histone modifications (H3K4me1, H3K4me3, H3K9me3, H3K27ac and H3K27me3) to their polyclonal counterparts in both human and mouse cells.

Results

Overall performance was highly similar for four monoclonal/polyclonal pairs, including when we used two

SUBMITTER: Busby M 

PROVIDER: S-EPMC5097419 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature