Enhanced cAMP-stimulated protein kinase A activity in human fibrolamellar hepatocellular carcinoma.

ABSTRACT:

Background

Fibrolamellar hepatocellular carcinoma (FL-HCC) affects children without underlying liver disease. A consistent mutation in FL-HCCs leads to fusion of the genes encoding a heat shock protein (DNAJB1) and the catalytic subunit of protein kinase A (PRKACA). We sought to characterize the resultant chimeric protein and its effects in FL-HCC.

Methods

The expression pattern and subcellular localization of protein kinase A (PKA) subunits in FL-HCCs were compared to paired normal livers by quantitative polymerase chain reaction (qPCR), immunoblotting, and immunofluorescence. PKA activity was measured by radioactive kinase assay, and we determined whether the FL-HCC mutation is present in other primary liver tumors.

Results

The fusion transcript and chimeric protein

SUBMITTER: Riggle KM 

PROVIDER: S-EPMC5105330 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature