Differential abilities of nitrogen dioxide and nitrite to nitrate proteins in thylakoid membranes isolated from Arabidopsis leaves.

ABSTRACT: Exposure of Arabidopsis leaves to nitrogen dioxide (NO₂) results in nitration of specific chloroplast proteins. To determine whether NO₂ itself and/or nitrite derived from NO₂ can nitrate proteins, Arabidopsis thylakoid membranes were isolated and treated with NO₂-bubbled or potassium nitrite (KNO₂) buffer, followed by protein extraction, electrophoresis, and immunoblotting using an anti-3-nitrotyrosine (NT) antibody. NO₂ concentrations in the NO₂-bubbled buffer were calculated by numerically solving NO₂ dissociation kinetic equations. The two buffers were adjusted to have identical nitrite concentrations. Both treatments yielded an NT-immunopositive band that LC/MS identified as PSBO1. The difference i

SUBMITTER: Takahashi M 

PROVIDER: S-EPMC5117089 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature