Insights into Nucleosome Organization in Mouse Embryonic Stem Cells through Chemical Mapping.

ABSTRACT: Nucleosome organization influences gene activity by controlling DNA accessibility to transcription machinery. Here, we develop a chemical biology approach to determine mammalian nucleosome positions genome-wide. We uncovered surprising features of nucleosome organization in mouse embryonic stem cells. In contrast to the prevailing model, we observe that for nearly all mouse genes, a class of fragile nucleosomes occupies previously designated nucleosome-depleted regions around transcription start sites and transcription termination sites. We show that nucleosomes occupy DNA targets for a subset of DNA-binding proteins, including CCCTC-binding factor (CTCF) and pluripotency factors. Furthermore, we provide evidence that promoter-proximal nucleosomes, with the +1 nucleosome in particular, con

SUBMITTER: Voong LN 

PROVIDER: S-EPMC5135608 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature