Global Analysis and Comparison of the Transcriptomes and Proteomes of Group A Streptococcus Biofilms.

ABSTRACT: To gain a better understanding of the genes and proteins involved in group A Streptococcus (GAS; Streptococcus pyogenes) biofilm growth, we analyzed the transcriptome, cellular proteome, and cell wall proteome from biofilms at different stages and compared them to those of plankton-stage GAS. Using high-throughput RNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) shotgun proteomics, we found distinct expression profiles in the transcriptome and proteome. A total of 46 genes and 41 proteins showed expression across the majority of biofilm time points that was consistently higher or consistently lower than that seen across the majority of planktonic time points. However, there was little overlap between the genes and proteins on these two li

SUBMITTER: Freiberg JA 

PROVIDER: S-EPMC5141267 | biostudies-literature | 2016 Nov-Dec

REPOSITORIES: biostudies-literature