Inhibition of de novo Methyltransferase 3B is a Potential Therapy for Hepatocellular Carcinoma.

ABSTRACT:

Background

Aberrant epigenetic patterns, including inactivation of tumor suppressor genes due to DNA methylation, have been described in many human cancers. Epigenetic therapeutic is a new and rapidly developing area of tumor treatment because DNA methyltransferase (DNMT) inhibitors can reverse its changes. We attempted to identify potential approach for epigenetic therapy of hepatocellular carcinoma.

Methods

We knocked down the expression of DNMT 1 or DNMT 3B by siRNA, and inhibited DNA methyltranferases by 5-Aza-2'-deoxycytidine. We used high-density oligonucleotide gene expression microarrays to examine the induced genes in human hepatocellular carcinoma cell line SMMC-7721 after suppressing DNA methyltranferases. The 5' ends of up-regulated genes were analyzed by BLAST d

SUBMITTER: Fan H 

PROVIDER: S-EPMC5154213 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature