DMSO Increases Mutation Scanning Detection Sensitivity of High-Resolution Melting in Clinical Samples.

ABSTRACT:

Background

Mutation scanning provides the simplest, lowest-cost method for identifying DNA variations on single PCR amplicons, and it may be performed before sequencing to avoid screening of noninformative wild-type samples. High-resolution melting (HRM) is the most commonly used method for mutation scanning. With PCR-HRM, however, mutations less abundant than approximately 3%-10% that can still be clinically significant may often be missed. Therefore, enhancing HRM detection sensitivity is important for mutation scanning and its clinical application.

Methods

We used serial dilution of cell lines containing the TP53 exon 8 mutation to demonstrate the improvement in detection sensitivity for conventional-PCR-HRM in the presence of DMSO. We also conducted coamplification at lo

SUBMITTER: Song C 

PROVIDER: S-EPMC5156568 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature