Direct identification of base-paired RNA nucleotides by correlated chemical probing.

ABSTRACT: Many RNA molecules fold into complex secondary and tertiary structures that play critical roles in biological function. Among the best-established methods for examining RNA structure are chemical probing experiments, which can report on local nucleotide structure in a concise and extensible manner. While probing data are highly useful for inferring overall RNA secondary structure, these data do not directly measure through-space base-pairing interactions. We recently introduced an approach for single-molecule correlated chemical probing with dimethyl sulfate (DMS) that measures RNA interaction groups by mutational profiling (RING-MaP). RING-MaP experiments reveal diverse through-space interactions corresponding to both secondary and tertiary structure. Here we develop a framework for using

SUBMITTER: Krokhotin A 

PROVIDER: S-EPMC5159650 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature