Gene expression variation between mouse inbred strains.

ABSTRACT:

Background

In this study, we investigated the effect of genetic background on expression profiles. We analysed the transcriptome of mouse hindlimb muscle of five frequently used mouse inbred strains using spotted oligonucleotide microarrays.

Results

Through ANOVA analysis with a false discovery rate of 10%, we show that 1.4% of the analysed genes is significantly differentially expressed between these mouse strains. Differential expression of several of these genes has been confirmed by quantitative RT-PCR. The number of genes affected by genetic background is approximately ten-fold lower than the number of differentially expressed genes caused by a dystrophic genetic defect.

Conclusions

We conclude that evaluation of the effect of background on gene expression profil

SUBMITTER: Turk R 

PROVIDER: S-EPMC516769 | biostudies-literature | 2004 Aug

REPOSITORIES: biostudies-literature