Application of next generation sequencing for the detection of human viral pathogens in clinical specimens.

ABSTRACT:

Background

Next generation sequencing (NGS) is a new technology that can be used for broad detection of infectious pathogens and is rapidly becoming an essential platform in clinical laboratories. It is not known how NGS will displace or enhance gold standard methodologies in infectious disease diagnosis.

Objectives

To investigate the feasibility and application of NGS technology in public health laboratories and compare NGS technology with conventional methods.

Study design

Illumina MiSeq system was used to detect viral pathogens alongside other conventional virology methods using typical clinical specimen matrices. Sixteen clinical specimens and two CDC proficiency panels containing seventeen specimens were analyzed.

Results

Known pathogenic viral nucleic aci

SUBMITTER: Parker J 

PROVIDER: S-EPMC5182090 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature