Next-Generation Sequencing for Characterizing Respiratory Tract Virome and Improving Detection of Viral Pathogens in Children With Pneumonia.

ABSTRACT:

Background

Pneumonia is typically caused by a variety of pathogenic microorganisms. Traditional research often focuses on the infection of a few microorganisms, whereas metagenomic studies focus on the impact of the bacteriome and mycobiome on respiratory diseases. Reports on the virome characteristics of pediatric pneumonia remain relatively scarce.

Methods

We employed de novo assembly and combined homology- and feature-based methods to characterize the respiratory virome in whole-genome DNA sequencing samples from oropharynx (OP) swabs, nasopharynx (NP) swabs, and bronchoalveolar lavage fluids (BALF) of children with pneumonia.

Results

Significant differences were observed in the alpha and beta diversity indexes, as well as in the composition of the oropharyngeal vi

SUBMITTER: Cui S 

PROVIDER: S-EPMC11310556 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature