Gender and developmental specific N-glycomes of the porcine parasite Oesophagostomum dentatum.

ABSTRACT:

Background

The porcine nodule worm Oesophagostomum dentatum is a strongylid class V nematode rather closely related to the model organism Caenorhabditis elegans. However, in contrast to the non-parasitic C. elegans, the parasitic O. dentatum is an obligate sexual organism, which makes both a gender and developmental glycomic comparison possible.

Methods

Different enzymatic and chemical methods were used to release N-glycans from male and female O. dentatum as well as from L3 and L4 larvae. Glycans were analysed by MALDI-TOF MS after either 2D-HPLC (normal then reversed phase) or fused core RP-HPLC.

Results

Whereas the L3 N-glycome was simpler and more dominated by phosphorylcholine-modified structures, the male and female worms express a wide range of core fucosylated

SUBMITTER: Jimenez-Castells C 

PROVIDER: S-EPMC5201199 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature