RELIC: a novel dye-bias correction method for Illumina Methylation BeadChip.

ABSTRACT:

Background

The Illumina Infinium HumanMethylation450 BeadChip and its successor, Infinium MethylationEPIC BeadChip, have been extensively utilized in epigenome-wide association studies. Both arrays use two fluorescent dyes (Cy3-green/Cy5-red) to measure methylation level at CpG sites. However, performance difference between dyes can result in biased estimates of methylation levels.

Results

Here we describe a novel method, called REgression on Logarithm of Internal Control probes (RELIC) to correct for dye bias on whole array by utilizing the intensity values of paired internal control probes that monitor the two color channels. We evaluate the method in several datasets against other widely used dye-bias correction methods. Results on data quality improvement showed that REL

SUBMITTER: Xu Z 

PROVIDER: S-EPMC5209853 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature