CD36 deficiency impairs the small intestinal barrier and induces subclinical inflammation in mice.

ABSTRACT:

Background & aims

CD36 has immuno-metabolic actions and is abundant in the small intestine on epithelial, endothelial and immune cells. We examined the role of CD36 in gut homeostasis using mice null for CD36 (CD36KO) and with CD36 deletion specific to enterocytes (Ent-CD36KO) or endothelial cells (EC-CD36KO).

Methods

Intestinal morphology was evaluated using immunohistochemistry and electron microscopy (EM). Intestinal inflammation was determined from neutrophil infiltration and expression of cytokines, toll-like receptors and COX-2. Barrier integrity was assessed from circulating lipopolysaccharide (LPS) and dextran administered intragastrically. Epithelial permeability to luminal dextran was visualized using two photon microscopy.

Results

The small intestines of CD

SUBMITTER: Cifarelli V 

PROVIDER: S-EPMC5217470 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature