Shotgun proteomics of the barley seed proteome.

ABSTRACT:

Background

Barley seed proteins are of prime importance to the brewing industry, human and animal nutrition and in plant breeding for cultivar identification. To obtain comprehensive proteomic data from seeds, total protein from a two-rowed (Conrad) and a six-rowed (Lacey) barley cultivar were precipitated in acetone, digested in-solution, and the resulting peptides were analyzed by nano-liquid chromatography coupled with tandem mass spectrometry.

Results

The raw mass spectra data searched against Uniprot's Barley database using in-house Mascot search engine identified 1168 unique proteins. Gene Ontology (GO) analysis indicated that the majority of the seed proteins were cytosolic, with catalytic activity and associated with carbohydrate metabolism. Spectral counting analysi

SUBMITTER: Mahalingam R 

PROVIDER: S-EPMC5219712 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature