Antigen Masking During Fixation and Embedding, Dissected.
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ABSTRACT:
SUBMITTER: Scalia CR
PROVIDER: S-EPMC5256198 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Publications
Antigen Masking During Fixation and Embedding, Dissected.
Scalia Carla Rossana CR Boi Giovanna G Bolognesi Maddalena Maria MM Riva Lorella L Manzoni Marco M DeSmedt Linde L Bosisio Francesca Maria FM Ronchi Susanna S Leone Biagio Eugenio BE Cattoretti Giorgio G
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 20161023 1
Antigen masking in routinely processed tissue is a poorly understood process caused by multiple factors. We sought to dissect the effect on antigenicity of each step of processing by using frozen sections as proxies of the whole tissue. An equivalent extent of antigen masking occurs across variable fixation times at room temperature. Most antigens benefit from longer fixation times (>24 hr) for optimal detection after antigen retrieval (AR; for example, Ki-67, bcl-2, ER). The transfer to a grade ...[more]
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