Generation of PDGFRα⁺ Cardioblasts from Pluripotent Stem Cells.

ABSTRACT: Isolating actively proliferating cardioblasts is the first crucial step for cardiac regeneration through cell implantation. However, the origin and identity of putative cardioblasts are still unclear. Here, we uncover a novel class of cardiac lineage cells, PDGFRα⁺Flk1^- cardioblasts (PCBs), from mouse and human pluripotent stem cells induced using CsAYTE, a combination of the small molecules Cyclosporin A, the rho-associated coiled-coil kinase inhibitor Y27632, the antioxidant Trolox, and the ALK5 inhibitor EW7197. This novel population of actively proliferating cells is cardiac lineage-committed but in a morphologically and functionally immature state compared to mature cardiomyocytes. Most important, most of CsAYTE-induced PCBs spontaneously differentiated into functional αMHC⁺ cardiomyocytes (M⁺CMs) and could be a potential cellular resource for cardiac regeneration.

SUBMITTER: Hong SP 

PROVIDER: S-EPMC5292955 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature