Platelet-activating factor (PAF) receptor as a promising target for cancer cell repopulation after radiotherapy.

ABSTRACT: A major drawback of radiotherapy is the accelerated growth of the surviving tumor cells. Radiotherapy generates a variety of lipids that bind to the receptor for platelet-activating factor, expressed by cells in the tumor microenvironment. In the present study, using the TC-1 tumor cell line, we found that irradiation induced a twofold increase in receptor expression and generated agonists of receptor. Irradiated cells induced a 20-fold increase in live TC-1 proliferation in vitro. Furthermore, subcutaneous co-injection of irradiated TC-1 cells with TC-1 expressing luciferase (TC-1 fluc⁺) markedly increased TC-1 fluc⁺ proliferation in a receptor-dependent way. Moreover we used a human carcinoma cell line not expressing the PAF receptor (KBM) and the same cell transfec

SUBMITTER: da Silva IA 

PROVIDER: S-EPMC5294253 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature