Identification of downy mildew resistance gene candidates by positional cloning in maize (Zea mays subsp. mays; Poaceae).

ABSTRACT:

Premise of the study

Positional cloning in combination with phenotyping is a general approach to identify disease-resistance gene candidates in plants; however, it requires several time-consuming steps including population or fine mapping. Therefore, in the present study, we suggest a new combined strategy to improve the identification of disease-resistance gene candidates.

Methods and results

Downy mildew (DM)-resistant maize was selected from five cultivars using a spreader row technique. Positional cloning and bioinformatics tools were used to identify the DM-resistance quantitative trait locus marker (bnlg1702) and 47 protein-coding gene annotations. Eventually, five DM-resistance gene candidates, including bZIP34, Bak1, and Ppr, were identified by q

SUBMITTER: Kim JY 

PROVIDER: S-EPMC5315382 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature