Ontology highlight
ABSTRACT:
SUBMITTER: Senturk S
PROVIDER: S-EPMC5322564 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Nature communications 20170222
The CRISPR/Cas9 system is a powerful tool for studying gene function. Here, we describe a method that allows temporal control of CRISPR/Cas9 activity based on conditional Cas9 destabilization. We demonstrate that fusing an FKBP12-derived destabilizing domain to Cas9 (DD-Cas9) enables conditional Cas9 expression and temporal control of gene editing in the presence of an FKBP12 synthetic ligand. This system can be easily adapted to co-express, from the same promoter, DD-Cas9 with any other gene of ...[more]