Ethylene production with engineered Synechocystis sp PCC 6803 strains.

ABSTRACT:

Background

Metabolic engineering and synthetic biology of cyanobacteria offer a promising sustainable alternative approach for fossil-based ethylene production, by using sunlight via oxygenic photosynthesis, to convert carbon dioxide directly into ethylene. Towards this, both well-studied cyanobacteria, i.e., Synechocystis sp PCC 6803 and Synechococcus elongatus PCC 7942, have been engineered to produce ethylene by introducing the ethylene-forming enzyme (Efe) from Pseudomonas syringae pv. phaseolicola PK2 (the Kudzu strain), which catalyzes the conversion of the ubiquitous tricarboxylic acid cycle intermediate 2-oxoglutarate into ethylene.

Results

This study focuses on Synechocystis sp PCC 6803 and shows stable ethylene production through the integration of a codon-optimize

SUBMITTER: Veetil VP 

PROVIDER: S-EPMC5324202 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature