Activated T Lymphocytes are Essential Drivers of Pathological Remodeling in Ischemic Heart Failure.

ABSTRACT:

Background

Inappropriately sustained inflammation is a hallmark of chronic ischemic heart failure (HF); however, the pathophysiological role of T lymphocytes is unclear.

Methods and results

Permanent coronary ligation was performed in adult C57BL/6 mice. When compared with sham-operated mice, mice with HF (8 weeks after ligation) exhibited the following features: (1) significant (P<0.05) expansion of circulating CD3⁺CD8⁺ cytotoxic and CD3⁺CD4⁺ helper (Th) T lymphocytes, together with increased Th1, Th2, Th17, and regulatory T-cell (Treg) CD4⁺ subsets; (2) significant expansion of CD8⁺ and CD4⁺ T cells in failing myocardium, with increased Th1, Th2, Th17, and Treg CD4⁺ subsets, marked reduction of the Th1/Th2 ratio, augmentation of the Th17/Treg ratio, and upregulation of Th2 cytokines; and (3) significantly increased Th1, Th2, Th17 cells, and Tregs, in the spleen and mediastinal lymph nodes, with expansion of splenic antigen-experienced effector and memory CD4⁺ T cells. Antibody-mediated CD4⁺ T-cell depletion in HF mice (starting 4 weeks after ligation) reduced cardiac infiltration of CD4⁺ T cells and prevented progressive left ventricular dilatation and hypertrophy, whereas adoptive transfer of splenic CD4⁺ T cells (and, to a lesser extent, cardiac CD3⁺ T cells) from donor mice with HF induced long-term left ventricular dysfunction, fibrosis, and hypertrophy in naive recipient mice.

Conclusions

CD4⁺ T lymphocytes are globally expanded and activated in chronic ischemic HF, with Th2 (versus Th1) and Th17 (versus Treg) predominance in failing hearts, and with expansion of memory T cells in the spleen. Cardiac and splenic T cells in HF are primed to induce cardiac injury and remodeling, and retain this memory on adoptive transfer.

SUBMITTER: Bansal SS 

PROVIDER: S-EPMC5331621 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature