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The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein.


ABSTRACT: The heterodimeric transcription elongation factor Spt4/Spt5 (Spt4/5) tightly associates with RNAPII to regulate both transcriptional elongation and co-transcriptional pre-mRNA processing; however, the mechanisms by which Spt4/5 acts are poorly understood. Recent studies of the human and Drosophila Spt4/5 complexes indicate that they can bind nucleic acids in vitro. We demonstrate here that yeast Spt4/5 can bind in a sequence-specific manner to single stranded RNA containing AAN repeats. Furthermore, we show that the major protein determinants for RNA-binding are Spt4 together with the NGN domain of Spt5 and that the KOW domains are not required for RNA recognition. These findings attribute a new function to a domain of Spt4/5 that associates directly with RNAPII, making significant steps towards elucidating the mechanism behind transcriptional control by Spt4/5.

SUBMITTER: Blythe AJ 

PROVIDER: S-EPMC5338239 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein.

Blythe Amanda J AJ   Yazar-Klosinski Berra B   Webster Michael W MW   Chen Eefei E   Vandevenne Marylène M   Bendak Katerina K   Mackay Joel P JP   Hartzog Grant A GA   Vrielink Alice A  

Protein science : a publication of the Protein Society 20160715 9


The heterodimeric transcription elongation factor Spt4/Spt5 (Spt4/5) tightly associates with RNAPII to regulate both transcriptional elongation and co-transcriptional pre-mRNA processing; however, the mechanisms by which Spt4/5 acts are poorly understood. Recent studies of the human and Drosophila Spt4/5 complexes indicate that they can bind nucleic acids in vitro. We demonstrate here that yeast Spt4/5 can bind in a sequence-specific manner to single stranded RNA containing AAN repeats. Furtherm  ...[more]

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