IL-32 induces indoleamine 2,3-dioxygenase⁺CD1c⁺ dendritic cells and indoleamine 2,3-dioxygenase⁺CD163⁺ macrophages: Relevance to mycosis fungoides progression.

ABSTRACT: Mycosis fungoides (MF) progresses from patch to tumor stage by expansion of malignant T-cells that fail to be controlled by protective immune mechanisms. In this study, we focused on IL-32, a cytokine, highly expressed in MF lesions. Depending on the other cytokines (IL-4, GM-CSF) present during in vitro culture of healthy volunteers' monocytes, IL-32 increased the maturation of CD11c⁺ myeloid dendritic cells (mDC) and/or CD163⁺ macrophages, but IL-32 alone showed a clear ability to promote dendritic cell (DC) differentiation from monocytes. DCs matured by IL-32 had the phenotype of skin-resident DCs (CD1c⁺), but more importantly, also had high expression of indoleamine 2,3-dioxygenase. The presence of DCs with these markers was demonstrated in MF sk

SUBMITTER: Ohmatsu H 

PROVIDER: S-EPMC5353917 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature