Comparative genomic hybridization using oligonucleotide microarrays and total genomic DNA.

ABSTRACT: Array-based comparative genomic hybridization (CGH) measures copy-number variations at multiple loci simultaneously, providing an important tool for studying cancer and developmental disorders and for developing diagnostic and therapeutic targets. Arrays for CGH based on PCR products representing assemblies of BAC or cDNA clones typically require maintenance, propagation, replication, and verification of large clone sets. Furthermore, it is difficult to control the specificity of the hybridization to the complex sequences that are present in each feature of such arrays. To develop a more robust and flexible platform, we created probe-design methods and assay protocols that make oligonucleotide microarrays synthesized in situ by inkjet technology compatible with array-based comparative geno

SUBMITTER: Barrett MT 

PROVIDER: S-EPMC535426 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature