A growing animal model for neonatal repair of large diaphragmatic defects to evaluate patch function and outcome.

ABSTRACT:

Objectives

We aimed to develop a more representative model for neonatal congenital diaphragmatic hernia repair in a large animal model, by creating a large defect in a fast-growing pup, using functional pulmonary and diaphragmatic read outs.

Background

Grafts are increasingly used to repair congenital diaphragmatic hernia with the risk of local complications. Growing animal models have been used to test novel materials.

Methods

6-week-old rabbits underwent fiberoptic intubation, left subcostal laparotomy and hemi-diaphragmatic excision (either nearly complete (n = 13) or 3*3cm (n = 9)) and primary closure (Gore-Tex patch). Survival was further increased by moving to laryngeal mask airway ventilation (n = 15). Sham operated animals were used as controls (n = 6). Surviv

SUBMITTER: Eastwood MP 

PROVIDER: S-EPMC5373533 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature