Novel micelle PCR-based method for accurate, sensitive and quantitative microbiota profiling.

ABSTRACT: In the last decade, many researchers have embraced 16S rRNA gene sequencing techniques, which has led to a wealth of publications and documented differences in the composition of microbial communities derived from many different ecosystems. However, comparison between different microbiota studies is currently very difficult due to the lack of a standardized 16S rRNA gene sequencing protocol. Here we report on a novel approach employing micelle PCR (micPCR) in combination with an internal calibrator that allows for standardization of microbiota profiles via their absolute abundances. The addition of an internal calibrator allows the researcher to express the resulting operational taxonomic units (OTUs) as a measure of 16S rRNA gene copies by correcting the number of sequences of each indivi

SUBMITTER: Boers SA 

PROVIDER: S-EPMC5381217 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature