Specificity, Privacy, and Degeneracy in the CD4 T Cell Receptor Repertoire Following Immunization.

ABSTRACT: T cells recognize antigen using a large and diverse set of antigen-specific receptors created by a complex process of imprecise somatic cell gene rearrangements. In response to antigen-/receptor-binding-specific T cells then divide to form memory and effector populations. We apply high-throughput sequencing to investigate the global changes in T cell receptor sequences following immunization with ovalbumin (OVA) and adjuvant, to understand how adaptive immunity achieves specificity. Each immunized mouse contained a predominantly private but related set of expanded CDR3β sequences. We used machine learning to identify common patterns which distinguished repertoires from mice immunized with adjuvant with and without OVA. The CDR3β sequences were deconstructed into sets of overlapping contigu

SUBMITTER: Sun Y 

PROVIDER: S-EPMC5390035 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature