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Effects of Mild and Severe Vitamin B₁ Deficiencies on the Meiotic Maturation of Mice Oocytes.

ABSTRACT: We investigated the effects of vitamin B₁ deficiency on the meiosis maturation of oocytes. Female Crl:CD1 (ICR) mice were fed a 20% casein diet (control group) or a vitamin B₁-free diet (test group). The vitamin B₁ concentration in ovary was approximately 30% lower in the test group than in the control group. Oocyte meiosis was not affected by vitamin B₁ deficiency when the deficiency was not accompanied by body weight loss. On the contrary, frequency of abnormal oocyte was increased by vitamin B₁ deficiency when deficiency was accompanied by body weight loss (referred to as severe vitamin B₁ deficiency; frequency of abnormal oocyte, 13.8% vs 43.7%, P = .0071). The frequency of abnormal oocytes was decreased by refeeding of a vitamin B₁-containing diet (13.9% vs 22.9%, P = .503). These results suggest that severe vitamin B₁ deficiency inhibited meiotic maturation of oocytes but did not damage immature oocytes.

SUBMITTER: Tsuji A

PROVIDER: S-EPMC5395269 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Effects of Mild and Severe Vitamin B1 Deficiencies on the Meiotic Maturation of Mice Oocytes.

Tsuji Ai A Nakamura Toshinobu T Shibata Katsumi K

Nutrition and metabolic insights 20170315

We investigated the effects of vitamin B1 deficiency on the meiosis maturation of oocytes. Female Crl:CD1 (ICR) mice were fed a 20% casein diet (control group) or a vitamin B1-free diet (test group). The vitamin B1 concentration in ovary was approximately 30% lower in the test group than in the control group. Oocyte meiosis was not affected by vitamin B1 deficiency when the deficiency was not accompanied by body weight loss. On the contrary, frequency ...[more]

PMID: 28469464

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