Germ Granules Prevent Accumulation of Somatic Transcripts in the Adult Caenorhabditis elegans Germline.

ABSTRACT: The germ cells of multicellular organisms protect their developmental potential through specialized mechanisms. A shared feature of germ cells from worms to humans is the presence of nonmembrane-bound, ribonucleoprotein organelles called germ granules. Depletion of germ granules in Caenorhabditis elegans (i.e., P granules) leads to sterility and, in some germlines, expression of the neuronal transgene unc-119::gfp and the muscle myosin MYO-3 Thus, P granules are hypothesized to maintain germ cell totipotency by preventing somatic development, although the mechanism by which P granules carry out this function is unknown. In this study, we performed transcriptome and single molecule RNA-FISH analyses of dissected P granule-depleted gonads at different developmenta

SUBMITTER: Knutson AK 

PROVIDER: S-EPMC5419467 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature