Detection and identification of the phytoplasma associated with China ixeris (Ixeridium chinense) fasciation.

ABSTRACT:

Background

Phytoplasmas are always associated with symptoms in host plants such as stunting of stems, witches'-broom, yellowing of leaves, formation of sterile-deformed flowers, virescence and phyllody. Recently also symptom of fasciation was reported associated with phytoplasma presence. In the present work, China ixeris fasciation was observed associated with phytoplasmas in Guanzhong Area, Shaanxi, China.

Results

Phytoplasma-like bodies were observed under transmission electron microscope in stem tissues of symptomatic samples. The 16S rRNA operon and tuf genes from phytoplasmas were amplified by PCR assays. Phylogenetic trees were calculated respectively based on sequences data of these two genes. The pattern of restriction fragment length polymorphism (RFLP) was generat

SUBMITTER: Li ZN 

PROVIDER: S-EPMC5430337 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature