Direct, rapid antimicrobial susceptibility test from positive blood cultures based on microscopic imaging analysis.

ABSTRACT: For the timely treatment of patients with infections in bloodstream and cerebrospinal fluid, a rapid antimicrobial susceptibility test (AST) is urgently needed. Here, we describe a direct and rapid antimicrobial susceptibility testing (dRAST) system, which can determine the antimicrobial susceptibility of bacteria from a positive blood culture bottle (PBCB) in six hours. The positive blood culture sample is directly mixed with agarose and inoculated into a micropatterned plastic microchip with lyophilized antibiotic agents. Using microscopic detection of bacterial colony formation in agarose, the total time to result from a PBCB for dRAST was only six hours for a wide range of bacterial concentrations in PBCBs. The results from the dRAST system were consistent with the results from a stand

SUBMITTER: Choi J 

PROVIDER: S-EPMC5430693 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature