A biochemical and biophysical model of G-quadruplex DNA recognition by positive coactivator of transcription 4.

ABSTRACT: DNA sequences that are guanine-rich have received considerable attention because of their potential to fold into a secondary, four-stranded DNA structure termed G-quadruplex (G4), which has been implicated in genomic instability and some human diseases. We have previously identified positive coactivator of transcription (PC4), a single-stranded DNA (ssDNA)-binding protein, as a novel G4 interactor. Here, to expand on these previous observations, we biochemically and biophysically characterized the interaction between PC4 and G4DNA. PC4 can bind alternative G4DNA topologies with a low nanomolar K_d value of ∼2 nm, similar to that observed for ssDNA. In consideration of the different structural features between G4DNA and ssDNA, these binding data indicated that PC4 can inter

SUBMITTER: Griffin WC 

PROVIDER: S-EPMC5465483 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature