Propofol induces excessive vasodilation of aortic rings by inhibiting protein kinase Cβ2 and θ in spontaneously hypertensive rats.

ABSTRACT:

Background and purpose

Exaggerated hypotension following administration of propofol is strongly predicted in patients with hypertension. Increased PKCs play a crucial role in regulating vascular tone. We studied whether propofol induces vasodilation by inhibiting increased PKC activity in spontaneously hypertensive rats (SHRs) and, if so, whether contractile Ca²⁺ sensitization pathways and filamentous-globular (F/G) actin dynamics were involved.

Experimental approach

Rings of thoracic aorta, denuded of endothelium, from normotensive Wistar-Kyoto (WKY) rats and SHR were prepared for functional studies. Expression and activity of PKCs in vascular smooth muscle (VSM) cells were determined by Western blot analysis and elisa respectively. Phosphorylation of the key pro

SUBMITTER: Wang Y 

PROVIDER: S-EPMC5466522 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature